Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancer/Testis Antigen 1B(CTAG1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cancer/Testis Antigen 1B(CTAG1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cancer/Testis Antigen 1B(CTAG1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cancer/Testis Antigen 1B(CTAG1B) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1; CAMEL; ESO2; LAGE-1; LAGE-2b; LAGE2A; Autoimmunogenic cancer/testis antigen NY-ESO-1; L antigen family member 2
Species：Human
Assay Type：Sandwich
Sensitivity：0.059 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates and other biological fluids
