Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Alpha(REG1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Alpha(REG1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Alpha(REG1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 1 Alpha(REG1a) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：REG; PSP; ICRF; PSPS1; PTP; Islet cells regeneration factor; Islet of Langerhans regenerating protein; Lithostathine 1 Alpha; Pancreatic Stone Protein; Pancreatic Thread Protein
Species：Mouse
Assay Type：Sandwich
Sensitivity：3.6 pg/mL
Standard：500 pg/mL
Detection range：7.82-500 pg/mL
Sample type：Serum, plasma and other biological fluids.
