Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Epsilon(IL1e). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Epsilon(IL1e). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Epsilon(IL1e), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Epsilon(IL1e) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：IL1F6; IL36a; FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1(EPSILON); Interleukin 36, Alpha; Interleukin 1 Family, Member 6
Species：Human
Assay Type：Sandwich
Sensitivity：2.7 pg/mL
Standard：500 pg/mL
Detection range：7.82-500 pg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
