Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na-Cl Cotransporter(NCCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Na-Cl Cotransporter(NCCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na-Cl Cotransporter(NCCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Na-Cl Cotransporter(NCCT) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SLC12A3; TSC; NCC; Solute Carrier Family 12 Member 3,Sodium/Chloride Transporters; Thiazide-sensitive sodium-chloride cotransporter; Na-Cl symporter
Species：Human
Assay Type：Sandwich
Sensitivity：0.115 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
