Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na-K-Cl Cotransporter 2(NKCC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Na-K-Cl Cotransporter 2(NKCC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na-K-Cl Cotransporter 2(NKCC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Na-K-Cl Cotransporter 2(NKCC2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter
Species：Human
Assay Type：Sandwich
Sensitivity：0.122 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
