Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma(NFYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Transcription Factor Y Gamma(NFYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Transcription Factor Y Gamma(NFYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Transcription Factor Y Gamma(NFYC) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CBF-C; NFYg; CBFC; H1TF2A; HAP5; HSM; NF-YC; hCBF-C; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; Transactivator HSM-1/2
Species：Human
Assay Type：Sandwich
Sensitivity：0.114 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids.
