Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stress Induced Phosphoprotein 1(STIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stress Induced Phosphoprotein 1(STIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stress Induced Phosphoprotein 1(STIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stress Induced Phosphoprotein 1(STIP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：HOP; P60; IEF-SSP-3521; STI1L; Hsp70/Hsp90-Organizing Protein; Renal carcinoma antigen NY-REN-11; Transformation-sensitive protein IEF SSP 3521
Species：Human
Assay Type：Sandwich
Sensitivity：0.63 ng/mL
Standard：100 ng/mL
Detection range：1.57-100 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
