Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 1(MRC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 1(MRC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 1(MRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Receptor C Type 1(MRC1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CD206; CLEC13D, CLEC13DL, MRC1L1; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1
Species：Mouse
Assay Type：Sandwich
Sensitivity：6.6 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates and other biological fluids
