Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-Motif Ligand 2(XCL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-Motif Ligand 2(XCL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-Motif Ligand 2(XCL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-Motif Ligand 2(XCL2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SCYC2; SCM-1b; Small Inducible Cytokine Subfamily C Member 2; Cytokine SCM-1 beta; C motif chemokine 2; XC chemokine ligand 2
Species：Human
Assay Type：Sandwich
Sensitivity：0.058 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Serum, plasma and other biological fluids.
