Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2(ALDH1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2(ALDH1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 1 Family, Member A2(ALDH1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 1 Family, Member A2(ALDH1A2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：RALDH(II); RALDH2; RALDH2-T; Retinaldehyde Dehydrogenase 2; Retinal dehydrogenase 2; Retinaldehyde-specific dehydrogenase type 2
Species：Human
Assay Type：Sandwich
Sensitivity：0.215 ng/mL
Standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
