Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin C Gamma(FLNC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin C Gamma(FLNC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin C Gamma(FLNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin C Gamma(FLNC) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：ABP-280; ABP280A; ABPA; ABPL; FLN2; Actin Binding Protein 280; ABP-280-like protein; Actin-binding-like protein; Filamin-2
Species：Human
Assay Type：Sandwich
Sensitivity：0.126 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
