Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prokineticin 2(PK2) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prokineticin 2(PK2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prokineticin 2(PK2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog
Species：Mouse
Assay Type：Competitive Inhibition
Sensitivity：91.3 pg/mL
Standard：20000 pg/mL
Detection range：312.5-20000 pg/mL
Sample type：Serum, plasma and other biological fluids
