Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tau-Protein Kinase 1(tPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tau-Protein Kinase 1(tPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tau-Protein Kinase 1(tPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tau-Protein Kinase 1(tPK1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：TTBK1; BDTK; TTK; Cdk5/P20; CDK5/P23; GSK; STK31; Brain-derived tau kinase; Tau-Tubulin Kinase 1; Tau-Protein O-Hosphotransferase; Brain Protein Kinase PK40erk
Species：Human
Assay Type：Sandwich
Sensitivity：0.056 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
