86-7835-48　Human GPI(Glucose 6 Phosphate Isomerase) ELISA Kit 96T　ELK1961

特徴

• Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Isomerase(GPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Isomerase(GPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Isomerase(GPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Isomerase(GPI) in the samples is then determined by comparing the OD of the samples to the standard curve.

仕様

• サイズ：96T
• Alternative Names：AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36
• Species：Human
• Assay Type：Sandwich
• Sensitivity：0.063 ng/mL
• Standard：10 ng/mL
• Detection range：0.16-10 ng/mL
• Sample type：Serum, plasma, tissue homogenates and other biological fluids
• Assay length：4.5h
• Research Area：
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