Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Delta-Sleep Inducing Peptide(dSIP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Delta-Sleep Inducing Peptide(dSIP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Delta-Sleep Inducing Peptide(dSIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：DSIPI; TSC22D3; DIP; GILZ; TSC-22R; hDIP; TSC22 Domain Family Protein 3; Glucocorticoid-Induced Leucine Zipper; Delta Sleep Inducing Peptide,Immunoreactor
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：7.15 pg/mL
Standard：1500 pg/mL
Detection range：23.44-1500 pg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
