Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen C Proteinase Enhancer 1(PCPE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen C Proteinase Enhancer 1(PCPE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen C Proteinase Enhancer 1(PCPE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen C Proteinase Enhancer 1(PCPE1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：PCOLCE; PCPE; Procollagen Type 1 COOH-Terminal Proteinase Enhancer; Procollagen C Endopeptidase Enhancer; Type I procollagen COOH-terminal proteinase enhancer
Species：Human
Assay Type：Sandwich
Sensitivity：0.285 ng/mL
Standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
