Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Crosslaps(bCTx) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Crosslaps(bCTx). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Crosslaps(bCTx) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Beta CTx，b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：37.5 pg/mL
Standard：8000 pg/mL
Detection range：125-8000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
