Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Peptide 1-40(Ab1-40). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amyloid Beta Peptide 1-40(Ab1-40). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Beta Peptide 1-40(Ab1-40), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amyloid Beta Peptide 1-40(Ab1-40) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Amyloid Beta Peptide 1-40
Species：Rat
Assay Type：Sandwich
Sensitivity：4.84 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma and other biological fluids
