Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C Receptor, Endothelial(PROCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C Receptor, Endothelial(PROCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C Receptor, Endothelial(PROCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C Receptor, Endothelial(PROCR) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CD201; CCCA ;CCD41; BA42O4.2; EPCR; Endothelial Protein C Receptor; Cell Cycle, Centrosome-Associated Protein; Activated Protein C Receptor
Species：Mouse
Assay Type：Sandwich
Sensitivity：34 pg/mL
Standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
