Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Receptor(C1qR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Receptor(C1qR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Receptor(C1qR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 1, Q Receptor(C1qR1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:CDw93; CD93; C1qRP; C1q-R; MXRA4; Matrix-remodeling-associated protein 4; CD93 Antigen; C1q/MBL/SPA receptor; Complement component 1 q subcomponent receptor 1
Species:Human
Assay Type:Sandwich
Sensitivity:6.7 pg/mL
Standard:1000 pg/mL
Detection range:15.63-1000 pg/mL
Sample type:serum, plasma and other biological fluids