Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transferrin(TRF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin(TRF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin(TRF) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
Species：Mouse
Assay Type：Competitive Inhibition
Sensitivity：5.78 ng/mL
Standard：1000 ng/mL
Detection range：15.63-1000 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
