Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B(MUC5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype B(MUC5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype B(MUC5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype B(MUC5B) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.239 ng/mL
Standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：tissue homogenates, cell lysates, cell culture supernates and other biological fluids
