86-7828-35　［取扱停止］Rat IgG(Immunoglobulin G) ELISA Kit 96T　ELK1254

特徴

• Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G(IgG) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G(IgG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G(IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.

仕様

• サイズ：96T
• Alternative Names：Immunoglobulin G
• Species：Rat
• Assay Type：Competitive Inhibition
• Sensitivity：0.49 μg/mL
• Standard：100 μg/mL
• Detection range：1.57-100 μg/mL
• Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay length：2h
• Research Area：
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