Molecular Weight:Approximately 17.4 kDa. The recombinant murine TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D.
Purity:> 98 % by SDS-PAGE and HPLC analyses.
Formulation:Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.