Description:The C5a ELISA Kit is for the in vitro determination of mouse C5a in different tissue samples including skin, muscle, nervous system and other biological samples. The microplate is coated with murine C5a monoclonal antibody. C5a present in the sample or standard binds to the antibodies absorbed to the microwells. Then, a biotin-conjugated anti-murine C5a antibody is added and binds to murine C5a captured by the first antibody. Next, streptavidin-horseradish peroxidase (HRP) is added, producing an antibody-antigen-antibody “sandwich”. Finally, substrate solution reactive with HRP is added to the well, and exhibits a blue color in direct proportion to the amount of C5a in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450 nm. The concentration of C5a in the samples is determined by comparing the O.D. of the samples to the standard curve.
仕様
Product Features:Sensitivity: The minimum detectable dose of mouse C5a is typically less than 7.8 pg/mL.Specificity: This assay recognizes recombinant and natural mouse C5a. No significant cross-reactivity or interference is observed.
