Description:Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed.3 ~30 μg transfection-grade plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit within 30 minutes.The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.
仕様
Components:
Buffer P1:15 mL
Buffer P2:15 mL
Buffer E3:15 mL
Buffer PS:15 mL
Buffer PW ( concentrate ):10 mL
Endotoxin-Free Buffer EB:10 mL
RNase A ( 10 mg/mL ):150 μL
Endotoxin-Remover Filter with collection tube:50 sets
Spin Column (Mini) with collection tube:50 sets
Application:
Sample:1 ~ 5mL E. Coli
Yield:3 ~ 30 μg EndoFree plasmid DNA
Elution:50 ~ 100 μL
Method:Spin Column
DNA Quality:Transfection, PCR, Enzyme digestion, Sequencing
