86-4619-67 Recombinant Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA),partial (Active) 20ug CSB-MP021334HU
[WUHAN HUAMEI BIOTECH Co.,Ltd(CUSABIO)]仕様
- Uniprot ID:P78324
- Research Topic:Cancer
- Gene Names:SIRPA
- Organism:Homo sapiens (Human)
- Source:Mammalian cell
- Expression Region:31-370aa
- Sequence Info:Partial
- Tag Info:C-terminal 6xHis-Myc-tagged
- AA Sequence:EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER
- MW:41.8 kDa
- Alternative Name(s):Brain Ig-like molecule with tyrosine-based activation motifs (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (Signal-regulatory protein alpha-2) (Sirp-alpha-2) (Signal-regulatory protein alpha-3) (Sirp-alpha-3) (p84) (CD_antigen: CD172a) (SHP substrate 1) (SHPS-1) (BIT) (MFR) (MYD1) (PTPNS1) (SHPS1) (SIRP)
- Purity:Greater than 95% as determined by SDS-PAGE.
- Biological_Activity:①Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 μg/ml can bind human CD47(CSB-MP004940HU), the EC50 of human SIRPA protein is 58.30-85.04 ng/ml.②Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
- Activity Image Link:https://www.cusabio.com/uploadfile/protein/1/CSB-MP021334HU-AC.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP021334HU-AC2.jpg
- Storage Buffer:Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
- Image Description:(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
- Endotoxin:Less than 1.0 EU/ug as determined by LAL method.
- Form:Lyophilized powder
- Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
- サイズ:20ug
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