86-4618-87　Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active) 100ug　CSB-MP004940HU

仕様

• Uniprot ID：Q08722
• Research Topic：Cancer
• Gene Names：CD47
• Organism：Homo sapiens （Human）
• Source：Mammalian cell
• Expression Region：19-139aa
• Sequence Info：Partial
• Tag Info：C-terminal hFc-tagged
• AA Sequence：QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP
• MW：41.5 kDa
• Alternative Name（s）：Antigenic surface determinant protein OA3（Integrin-associated protein）（IAP）（Protein MER6）（CD47）
• Purity：Greater than 95％ as determined by SDS-PAGE.
• Biological_Activity：①Measured by its binding ability in a functional ELISA. Immobilized SIRPA （CSB-MP021334HU） at 2 μg/ml can bind human CD47, the EC50 of human CD47 protein is 65.91-82.42 ng/ml.②Human SIRPA protein His/Myc tag （CSB-MP021334HU） captured on COOH chip can bind Human CD47 protein Fc tag （CSB-MP004940HU） with an affinity constant of 19.1 nM as detected by LSPR Assay.
• Activity Image Link：https://www.cusabio.com/uploadfile/protein/1/CSB-MP004940HU-AC.jpg
• Storage Buffer：Lyophilized from a 0.2 μm filtered PBS, 6％ Trehalose, pH 7.4
• Image Description：（Tris-Glycine gel） Discontinuous SDS-PAGE （reduced） with 5％ enrichment gel and 15％ separation gel.
• Endotoxin：Less than 1.0 EU/ug as determined by LAL method.
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：100ug
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