86-4597-45　Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active) 20ug　CSB-MP866317HU

仕様

• Uniprot ID：Q9BYF1
• Research Topic：Cell Biology
• Gene Names：ACE2
• Organism：Homo sapiens （Human）
• Source：Mammalian cell
• Expression Region：18-740aa
• Sequence Info：Partial
• Tag Info：C-terminal hFc-tagged
• AA Sequence：QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
• MW：112.5 kDa
• Alternative Name（s）：ACE 2; ACE related carboxypeptidase; ACE-related carboxypeptidase; ACE2; ACE2_HUMAN; ACEH; Angiotensin converting enzyme 2; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme （peptidyl dipeptidase A） 2; Angiotensin I converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZP434A014; EC 3.4.17; metalloprotease MPROT 15; Metalloprotease MPROT15; OTTHUMP00000022963; Processed angiotensin-converting enzyme 2
• Purity：Greater than 85％ as determined by SDS-PAGE.
• Biological_Activity：①Measured by ACE2 protein （CSB-MP866317HU） binding ability in a functional ELISA.Coat the plate with 0.2μg/well （2μg/ml, 100μl/well） of SARS-CoV-2 S1 protein （CSB-MP3324GMY）, add 100μl/well （0.7629-100000ng/ml, gradient dilution, 2x to 18x） of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific （CSB-PA160506）. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml.②Measured by ACE2 protein （CSB-MP866317HU） binding ability in a functional ELISA.Coat the plate with 0.5μg/well （5μg/ml, 100μl/well） of SARS-CoV-2 S1-RBD protein （CSB-YP3324GMY1）, add 100μl/well （0.762939-100000ng/ml, gradient dilution, 2x to 18x） of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific （CSB-PA160506）. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml.③Measured by ACE2 protein （CSB-MP866317HU） binding ability in a functional ELISA.Coat the plate with 0.5μg/well （5μg/ml, 100μl/well） of SARS-CoV-2 S1-RBD protein （CSB-MP3324GMY1b1）, add 100μl/well （0.762939-100000ng/ml, gradient dilution, 2x to 18x） of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific （CSB-PA160506）. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml.④Measured by ACE2 protein （CSB-MP866317HU） binding ability in a functional ELISA.Coat the plate with 0.2μg/well （2μg/ml, 100μl/well） of ACE2 protein, add 100μl/well （0.00001164153-200000ng/ml, gradient dilution, 4x to 18x） of SARS-CoV-2 S1-RBD protein （CSB-MP3324GMY1） and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific （CSB-PA160506）. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml.⑤The SPR sensorgram shows the binding between ACE2 protein （CSB-MP866317HU） and SARS-CoV-2 S1-RBD protein （CSB-YP3324GMY1）.ACE2 protein was injected at 5 concentrations （400, 200, 100, 50, 0 nM） across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM.⑥The SPR sensorgram shows the binding between ACE2 protein （CSB-MP866317HU） and SARS-CoV-2 S1-RBD protein （CSB-MP3324GMY1b1）.ACE2 protein was injected at 5 concentrations （100, 50, 25, 12.5, 0 nM） across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
• Activity Image Link：https://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC2.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC3.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC4.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC5.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP866317HU-AC6.jpg
• Storage Buffer：Lyophilized from Tris/PBS-based buffer, 6％ Trehalose, pH 8.0
• Image Description：（Tris-Glycine gel） Discontinuous SDS-PAGE （reduced） with 5％ enrichment gel and 15％ separation gel.
• Endotoxin：Less than 1.0 EU/ug as determined by LAL method.
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：20ug
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