86-4596-13　Recombinant Human SARS coronavirus Spike glycoprotein(S),partial (Active) 1mg　CSB-MP348663HQE

仕様

• Uniprot ID：P59594
• Research Topic：Cancer
• Gene Names：S
• Organism：Human SARS coronavirus （SARS-CoV） （Severe acute respiratory syndrome coronavirus）
• Source：Mammalian cell
• Expression Region：306-527aa
• Sequence Info：Partial
• Tag Info：N-terminal 10xHis-tagged and C-terminal Myc-tagged
• AA Sequence：RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF
• MW：30 kDa
• Alternative Name（s）：（S glycoprotein）（E2）（Peplomer protein）（Spike protein S1）（Spike protein S2）
• Purity：Greater than 95％ as determined by SDS-PAGE.
• Biological_Activity：①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 （CSB-MP684964PAL）, the EC50 is 5.056-7.559 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 （CSB-MP866317HU）, the EC50 is 7.941-10.49 ng/ml.
• Activity Image Link：https://www.cusabio.com/uploadfile/protein/1/CSB-MP348663HQE-AC1.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP348663HQE-AC2.jpg
• Storage Buffer：Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6％ Trehalose, pH 8.0
• Image Description：（Tris-Glycine gel） Discontinuous SDS-PAGE （reduced） with 5％ enrichment gel and 15％ separation gel.
• Endotoxin：Less than 1.0 EU/ug as determined by LAL method.
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：1mg
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