86-4595-79　Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active) 1mg　CSB-MP3324GMY1b1

仕様

• Uniprot ID：P0DTC2
• Research Topic：Microbiology
• Gene Names：S
• Organism：Severe acute respiratory syndrome coronavirus 2 （2019-nCoV） （SARS-CoV-2）
• Source：Mammalian cell
• Expression Region：319-541aa
• Sequence Info：Partial
• Tag Info：N-terminal 10xHis-tagged and C-terminal Myc-tagged
• AA Sequence：RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
• MW：30.1 kDa
• Alternative Name（s）：S glycoprotein;E2;Peplomer protein
• Purity：Greater than 90％ as determined by SDS-PAGE.
• Biological_Activity：①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody （CSB-RA33245A1GMY）, the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody （CSB-RA33245A0GMY）, the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 （CSB-MP866317HU）, the EC50 is 2.785-9.139 ng/ml.④SARS-CoV-2 Spike protein RBD his/myc tag （CSB-MP3324GMY1b1） captured on COOH chip can bind Human ACE2 protein Fc tag （CSB-MP866317HU） with an affinity constant of 13.8 nM as detected by LSPR Assay.⑤Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 （CSB-MP866317HU-B）, the EC50 is 4.599-8.322 ng/ml.
• Activity Image Link：https://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1b1-AC1.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1b1-AC2.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1b1-AC3.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1b1-AC4.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1b1-AC5.jpg
• Storage Buffer：Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6％ Trehalose, pH 8.0
• Image Description：（Tris-Glycine gel） Discontinuous SDS-PAGE （reduced） with 5％ enrichment gel and 15％ separation gel. Predicted band size： 30.1 kDaObserved band size： 35 kDa due to glycosylation
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：1mg
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