86-4595-41　Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial (Active) 100ug　CSB-MP3324GMY1

仕様

• Uniprot ID：P0DTC2
• Research Topic：Microbiology
• Gene Names：S
• Organism：Severe acute respiratory syndrome coronavirus 2 （2019-nCoV） （SARS-CoV-2）
• Source：Mammalian cell
• Expression Region：319-541aa
• Sequence Info：Partial
• Tag Info：C-terminal 6xHis-mFc-tagged
• AA Sequence：RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
• MW：51.1 kDa
• Alternative Name（s）：Spike glycoprotein（RBD）,Spike Protein （RBD）
• Purity：Greater than 90％ as determined by SDS-PAGE.
• Biological_Activity：①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody （CSB-RA33245D1GMY）, the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human ACE2 （CSB-MP866317HU） at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
• Activity Image Link：https://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1-AC1.jpghttps://www.cusabio.com/uploadfile/protein/1/CSB-MP3324GMY1-AC2.jpg
• Storage Buffer：Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6％ Trehalose, pH 8.0
• Image Description：（Tris-Glycine gel） Discontinuous SDS-PAGE （reduced） with 5％ enrichment gel and 15％ separation gel. Predicted band size： 51.1 kDaObserved band size： 66 kDa due to glycosylation
• Endotoxin：Less than 1.0 EU/ug as determined by LAL method.
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：100ug
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