86-4486-71　［取扱停止］Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active) 1mg　CSB-AP000101HU

仕様

• Uniprot ID：P23368
• Research Topic：Signal Transduction
• Gene Names：ME2
• Organism：Homo sapiens （Human）
• Source：E.Coli
• Expression Region：19-584aa
• Sequence Info：Full Length of Mature Protein
• Tag Info：C-terminal 6xHis-tagged
• AA Sequence：MLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITE+HHHHHH
• MW：64.4 kDa
• Alternative Name（s）：NAD-ME, Malic enzyme 2
• Purity：＞95％ as determined by SDS-PAGE and HPLC.
• Biological_Activity：Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer （1975）. The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
• Storage Buffer：Lyophilized from a 0.2μm filtered PBS, pH 7.4.
• Endotoxin：Less than 1.0 EU/μg as determined by LAL method.
• Form：Lyophilized powder
• Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50％ of glycerol （final concentration） and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50％. Customers could use it as reference.
• サイズ：1mg
• この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）