Background:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
仕様
Synonyms:Aging associated gene 9 protein,Epididymis secretory sperm binding protein Li 162eP,G3P,G3PD,G3PDH,GAPD,GAPDH,Glyceraldehyde 3 phosphate dehydrogenase,Glyceraldehyde-3-phosphate dehydrogenase,HEL S 162eP,Peptidyl-cysteine S-nitrosylase GAPDH
Host:Rabbit
Reactivity:Zebrafish
Applications:WB
Concentration:1mg/mL
Immunogen:Recombinant Protein
Purification Method:Affinity purification
Clonality:Polyclonal
Conjugation:Unconjugated
Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Dilution:WB 1:2000-5000
Gene_ID(human):2597
Swissprot:P04406
Isotype:IgG
Research Areas:Cancer, Metabolism, Signal transduction