Background：Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining （NHEJ） required for double-strand break repair and V（D）J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase （5'-dRP lyase）, by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
仕様
Synonyms：5''-deoxyribose-5-phosphate lyase Ku70,5''-dRP lyase Ku70,70 kDa subunit of Ku antigen,ATP dependent DNA helicase 2 subunit 1,ATP dependent DNA helicase II 70 kDa subunit,ATP-dependent DNA helicase 2 subunit 1,ATP-dependent DNA helicase II 70 kDa subunit,CTC box binding factor 75 kDa subunit,CTC box-binding factor 75 kDa subunit,CTC75,CTCBF,DNA repair protein XRCC6,G22P1,Ku 70,Ku autoantigen p70 subunit,Ku autoantigen,70kDa,Ku p70,Ku70,Ku70 DNA binding component of DNA-dependent proteinkinase complex （thyroid autoantigen 70 kDa,Kup70,Lupus Ku autoantigen protein p70,ML8,Thyroid autoantigen 70kD （Ku antigen）,Thyroid autoantigen,Thyroid lupus autoantigen,Thyroid lupus autoantigen p70,Thyroid-lupus autoantigen,TLAA,X ray repair complementing defective repair in Chinese hamster cells 6,X-ray repair complementing defective repair in Chinese hamster cells 6,X-ray repair cross-complementing protein 6,XRCC 6,Xrcc6,XRCC6
Host：Rabbit
Reactivity：Human,Mouse,Rat
Applications：WB,ELISA
Concentration：1mg/mL
Immunogen：Synthetic Acetyl peptide from human protein
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide and 50％ glycerol pH 7.4.
Dilution：WB 1：500-2000, ELISA 1：10000-20000
Gene_ID（human）：2547
Swissprot：P12956
Isotype：IgG
Research Areas：Cancer, Epigenetics and Nuclear Signaling
