Background：This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3＆apos; splice site, together with the large subunit of U2 auxiliary factor （U2AF）, and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
仕様
Synonyms：BBP,D11S636,Mammalian branch point binding protein mBBP,OTTHUMP00000069749,OTTHUMP00000069750,OTTHUMP00000069752,Splicing factor 1,Transcription factor ZFM1,ZFM1,Zinc finger gene in MEN1 locus,Zinc finger protein 162,ZNF162
Host：Rabbit
Reactivity：Human,Mouse,Monkey
Applications：WB,IHC-p,IF,ELISA
Concentration：1mg/mL
Immunogen：Synthesized peptide derived from human Splicing factor 1 around the phosphorylation site of S82.
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide,0.5％ BSA and 50％ glycerol pH 7.4.
Dilution：WB 1：500-1：2000, IHC 1：100-1：300, IF 1：200-1：1000, ELISA 1：5000
