Background：Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB （p50 and p65） and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kappaB sequence in the immunoglobulin kappa light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkappaB is activated and NFkappaB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
仕様
Synonyms：Avian reticuloendotheliosis viral （v rel） oncogene homolog A,MGC131774,NF kappa B p65delta3,NFKB3,Nuclear Factor NF Kappa B p65 Subunit,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B cells 3,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,OTTHUMP00000233473,OTTHUMP00000233474,OTTHUMP00000233475,OTTHUMP00000233476,OTTHUMP00000233900,p65,p65 NF kappaB,p65 NFkB,relA,TF65,Transcription factor p65,v rel avian reticuloendotheliosis viral oncogene homolog A （nuclear factor of kappa light polypeptide gene enhancer in B cells 3 （p65））,V rel avian reticuloendotheliosis viral oncogene homolog A,v rel reticuloendotheliosis viral oncogene homolog A （avian）,V rel reticuloendotheliosis viral oncogene homolog A,nuclear factor of kappa light polypeptide gene enhancer in B cells 3,p65
Host：Rabbit
Reactivity：Human,Mouse,Rat,Monkey
Applications：WB,IHC-p,IF,ELISA
Concentration：1mg/mL
Immunogen：Synthesized peptide derived from human NFkappaB-p65 around the phosphorylation site of Ser529
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide, 0.5％ BSA and 50％ glycerol, pH7.4
Dilution：WB 1：500-1：2000, IHC 1：100-1：300, IF 1：50-1：200, ELISA 1：10000-1：20000
Gene_ID（human）：5970
Swissprot：Q04206
Isotype：IgG
Research Areas：Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal transduction
