Background：Ubiquitin exists either covalently attached to another protein, or free （unanchored）. When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer （monoubiquitin）, a polymer linked via different Lys residues of the ubiquitin （polyubiquitin chains） or a linear polymer linked via the initiator Met of the ubiquitin （linear polyubiquitin chains）. Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked： Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD （endoplasmic reticulum-associated degradation） and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling.
仕様
Synonyms：UBB,Polyubiquitin-B,UBC,Polyubiquitin-C,RPS27A,UBA80,UBCEP1,Ubiquitin-40S ribosomal protein S27a,Ubiquitin carboxyl extension protein 80,UBA52,UBCEP2,Ubiquitin-60S ribosomal protein L40,CEP52,Ubiquitin A-52 residue ribosomal protein fusion product 1
Host：Rabbit
Reactivity：Human,Mouse,Rat
Applications：WB,ELISA
Concentration：1mg/mL
Immunogen：Synthesized peptide derived from the human Ub around the acetylation site of K29.
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide, 0.5％ BSA and 50％ glycerol, pH7.4
Dilution：WB 1：500-1：2000, ELISA 1：20000
Swissprot：P62987,P62979,P0CG47,P0CG48
Isotype：IgG
Research Areas：Cancer, Cell Biology, Epigenetics and Nuclear Signaling
