Background：NADH：ubiquinone oxidoreductase （complex I） is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by trans-location of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes （subunits 1-7）, while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 3 （ND3） localizes to the hydrophobic protein fragment of complex I. Mutations in the gene encodiing for ND3 may be associated with Parkinson disease.
