Background：PICH （Plk1-interacting checkpoint helicase）, also known as DNA excision repair protein ERCC-6-like （ERCC6L） or tumor antigen BJ-HCC-15, is a 1,250 amino acid protein belonging to the SNF2/RAD54 helicase family. PICH is a DNA helicase and an essential component of the spindle assembly checkpoint. During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin. PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores. PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region. PICH/Plk interaction is also required for correct Plk localization to the kinetochore. PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.
仕様
Synonyms：ATP dependent helicase ERCC6 like,ATP-dependent helicase ERCC6-like,DNA excision repair protein ERCC 6 like,DNA excision repair protein ERCC-6-like,ERC6L,ERCC 6L,Ercc6l,Excision repair cross complementing rodent repair deficiency complementation group 6 like,Excision repair protein ERCC6 like,FLJ20105,MGC131695,PICH,Plk1 interacting checkpoint helicase,PLK1-interacting checkpoint helicase,SNF2/RAD54 family protein,Tumor antigen BJ HCC 15,Tumor antigen BJ-HCC-15
Host：Rabbit
Reactivity：Human
Applications：IHC,ELISA
Concentration：0.5mg/mL
Immunogen：Recombinant protein of human ERCC6L
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.05％ sodium azide, 50％ glycerol, PH7.3
Dilution：IHC 1：50-1：200
Swissprot：Q2NKX8
Isotype：IgG
Research Areas：Cell Biology, Epigenetics and Nuclear Signaling
