Background：Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor （HIF） alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation （ODD） domains （N-terminal, NODD, and C-terminal, CODD） of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs （particularly HIF2A） in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex.
仕様
Synonyms：Egl 9 family hypoxia inducible factor 3,Egl nine homolog 3 （C. elegans）,Egl nine homolog 3,Egl nine like protein 3 isoform,EGL9 homolog of C. elegans 3,EGLN3,EGLN3,Factor responsive smooth muscle protein,HIF Prolyl Hydroxylase 3,HIF-PH3,HIF-prolyl hydroxylase 3,HIFP4H3,HIFPH3,HPH-1,HPH-3,Hypoxia-inducible factor prolyl hydroxylase 3,P4H3,PHD3,Prolyl Hydroxylase Domain Containing Protein 3,Prolyl hydroxylase domain-containing protein 3,SM20
Host：Rabbit
Reactivity：Human,Mouse,Rat
Applications：IHC,ELISA
Concentration：0.4mg/mL
Immunogen：Recombinant protein of human EGLN3
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.05％ sodium azide, 50％ glycerol, PH7.3
Dilution：IHC 1：25-1：100
Swissprot：Q9H6Z9
Isotype：IgG
Research Areas：Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
