Background：MGMT （O6-methylguanine-DNA methyltransferase） is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.
仕様
Synonyms：6 O methylguanine DNA methyltransferase,6-O-methylguanine-DNA methyltransferase,Agat,AGT,AI267024,EC 2.1.1.63,Methylated DNA protein cysteine methyltransferase,Methylated-DNA--protein-cysteine methyltransferase,Methylguanine DNA methyltransferase,MGC107020,MGMT,MGMT,O 6 methylguanine DNA alkyltransferase,O 6 methylguanine DNA methyltransferase,O-6-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase
Host：Rabbit
Reactivity：Human
Applications：WB,ELISA
Concentration：0.1mg/mL
Immunogen：Recombinant protein of human MGMT
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.05％ sodium azide, 50％ glycerol, PH7.3
Dilution：WB 1：200-1：1000
Swissprot：P16455
Isotype：IgG
Research Areas：Cancer, Epigenetics and Nuclear Signaling
