Background：Substrate recognition component of a SCF （SKP1-CUL1-F-box protein） E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
仕様
Synonyms：CDK2/Cyclin A associated protein p45,Cyclin A/CDK2 associated protein p45,Cyclin-A/CDK2-associated protein p45,F box protein Skp2,F box/LRR repeat protein 1,F-box protein Skp2,F-box/LRR-repeat protein 1,FBL 1,FBL1,FBXL 1,FBXL1,FLB 1,FLB1,MGC1366,p45,p45skp2,S phase kinase associated protein 2 （p45）,S phase kinase associated protein 2,S-phase kinase-associated protein 2,S-phase kinase-associated protein 2 E3 ubiquitin protein ligase,SKP 2,Skp2,SKP2
Host：Rabbit
Reactivity：Human
Applications：WB,IHC-p,IF,ELISA
Concentration：1mg/mL
Immunogen：Synthesized peptide derived from the C-terminal region of human Skp2 p45
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide, 0.5％ BSA and 50％ glycerol, pH7.4
Dilution：WB 1：500-1：2000, IHC 1：100-1：300, IF 1：200-1：1000, ELISA 1：20000
Gene_ID（human）：6502
Swissprot：Q13309
Isotype：IgG
Research Areas：Cancer, Cell Biology, Cardiovascular
