Background：Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
仕様
Synonyms：PTTG1,EAP1,PTTG,TUTR1,Securin,Esp1-associated protein,Pituitary tumor-transforming gene 1 protein,Tumor-transforming protein 1,hPTTG
Host：Rabbit
Reactivity：Human
Applications：WB,IHC-p,IF,ELISA
Concentration：1mg/mL
Immunogen：Synthesized peptide derived from the Internal region of human PTTG1/2/3
Purification Method：Affinity purification
Clonality：Polyclonal
Conjugation：Unconjugated
Buffer：PBS with 0.02％ sodium azide, 0.5％ BSA and 50％ glycerol, pH7.4
Dilution：WB 1：500-1：2000, IHC 1：100-1：300, IF 1：200-1：1000, ELISA 1：20000
Gene_ID（human）：9232
Swissprot：O95997
Isotype：IgG
Research Areas：Cancer, Cell Biology, Epigenetics and Nuclear Signaling
