背景：Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
仕様
種由来：MERS-CoV
発現宿主：Baculovirus-Insect Cells
純度：> 90 % as determined by SDS-PAGE
遺伝子構造：A DNA sequence encoding the Nucleoprotein MERS-CoV (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus.
エンドトキシン濃度：< 1.0 EU per μg of the protein as determined by the LAL method
分子量：46.51kDa.
組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual.
保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
