背景：Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
仕様
種由来：HCoV-OC43
発現宿主：E. coli
純度：> 90 % as determined by SDS-PAGE.
遺伝子構造：A DNA sequence encoding the Human coronavirus (HCoV-OC43) Nucleoprotein (YP_009555245.1) (Met1-Ile448) was expressed with a polyhistide tag at the N-terminus.
アミノ酸配列：Met1-Ile448
アクセッションＮｏ．：YP_009555245.1
タグ種類：N-His
エンドトキシン濃度：Please contact us for more information.
分子量：50.43KDa.
組成：Lyophilized from sterile 40mM PB, 500mM NaCl, pH 7.4Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual.
保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
