背景：Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
仕様
種由来：ZIKV
発現宿主：Baculovirus-Insect Cells
純度：> 90 % as determined by SDS-PAGE.
遺伝子構造：A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1, with mutation Thr 356 Ala, Gln 357 Gly, Trp 381 Arg, Leu 387 Arg) (Ile291-Thr696) was expressed with a polyhistidine tag at the C-terminus.
別名（遺伝子名）：Zika virus, E protein, strain Zika SPH2015
アクセッションＮｏ．：ALU33341.1
タグ種類：C-His
エンドトキシン濃度：< 1.0 EU per μg protein as determined by the LAL method.
分子量：45.6kDa.
組成：Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual.
保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
