86-4051-76 ［取扱停止］Recombinant Active Glycerol kinase Protein (His Tag) 100ug PKSQ500004
[Elabscience]
特徴
- 背景：Glycerol kinase from E. coli (glpK) catalyzes the ATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate (G3P), the first and rate-limiting step in the utilization of glycerol. In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator. In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6-bisphosphate (FBP) of the glycolytic pathway. Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer. FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway. GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70. Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism. The enzyme activity was measured using a phosphatase-coupled kinase assay.
仕様
- 種由来：Escherichia coli (strain K12)
- 発現宿主：E.coli
- 純度：>90 % as determined by reducing SDS-PAGE.
- 遺伝子構造：A DNA sequence encoding the Escherichia coli Glycerol kinase (2T-502E) was expressed with a polyhistidine tag at the N-terminus and C-terminus.
- 別名（遺伝子名）：GK, glpK
- アクセッションＮｏ．：P0A6F3
- タグ種類：N/C-His
- エンドトキシン濃度：Not tested.
- 分子量：56.1kDa
- 組成：Lyophilized from sterile potassium phosphate, pH 7.4
- 保存条件：Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- メーカーマニュアルリンク：https://u2.elabscience.com/viewpdf-463618-elabscience-PKSQ500004.pdf
- サイズ：100ug
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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86-4051-76
Recombinant Active Glycerol kinase Protein (His Tag) 100ug
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86-4054-65
Recombinant Active Glycerol kinase Protein (His Tag) 400ug
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