特徴
- 背景：Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
仕様
- 種由来：Human
- 発現宿主：E.coli
- 純度：>95% as determined by reducing SDS-PAGE.
- 遺伝子構造：Recombinant Human Bromodomain-containing protein 4 is produced by our E.coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, Flag tag at the N-terminus.
- 別名（遺伝子名）：HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP
- アミノ酸配列：Glu49-Glu460
- アクセッションＮｏ．：O60885
- タグ種類：N-10His-Flag
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- 分子量：49 kDa
- 組成：Supplied as a 0.2 μm filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
- 保存条件：Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://u2.elabscience.com/viewpdf-463446-elabscience-PKSH033982.pdf
- サイズ：10ug
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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86-4051-11
Recombinant Human BRD4 (N-10His-Flag) 50ug
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86-4046-93
Recombinant Human BRD4 (N-10His-Flag) 10ug
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