特徴
- 背景：Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
仕様
- 種由来：Human
- 発現宿主：Human Cells
- 純度：>90% as determined by reducing SDS-PAGE.
- 遺伝子構造：Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17Thr546 is expressed with a mFc tag at the C-terminus.
- 別名（遺伝子名）：SIGLEC10; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; Siglec-10;siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
- アミノ酸配列：Met17-Thr546
- アクセッションＮｏ．：Q96LC7
- タグ種類：C-mFc
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- 分子量：84.6 kDa
- 組成：Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://u2.elabscience.com/viewpdf-463375-elabscience-PKSH033911.pdf
- サイズ：10ug
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Recombinant Human Siglec-10 (C-mFc) 10ug
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Recombinant Human Siglec-10 (C-mFc) 50ug
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